Services

NGS

Amplicon Metagenomic Sequencing Shotgun Metagenomic Sequencing Full Length 16S rRNA Sequencing
Amplicon Metagenomic Sequencing

Amplicon Metagenomic Sequencing

Amplicon metagenomic sequencing is an efficient and economical sequencing method for identifying and comparing bacteria, fungi, and other microorganisms. NGS-based analysis allows for taxonomic comparisons of complex microbiomes or cluster diversities within an environment that are difficult or impossible to culture and provides visual and statistical indicators to aid researchers' understanding. If Amplicon sequencing is not using a universal primer set, sufficient consultation is required for experimentation and analysis.

Application

  • Taxonomic profiling
  • Comparision analysis of microbial community
  • Diversity statistics (alpha-, beta- diversity)

Sample/Laboratory Information

Sample/Laboratory Information
Sample Requirement gDNA > 100ng (minimum 10ng) 20ng/µl DIN > 6
Library Kit Illumina
Sequencing Platform Illumina MiSeq
Recommended Sequencing Depth Up to 50,000 reads per sample

Bioinformatics

Workflow

Standard Analysis

Pick De novo OTUs

Microbioal taxonomic Profiling

Diversity statistics(alpha-, beta- diversity)

Standard Analysis

Reference

    [1] Lim, M. Y. et al., (2020). Changes in microbiome and metabolomic profiles of fecal samples stored with stabilizing solution at room temperature: a pilot study. Scientific reports, 10(1), 1789.

    [2] Gunathilake, M. N. et al., (2019). Association between the relative abundance of gastric microbiota and the risk of gastric cancer: a case-control study. Scientific reports, 9(1), 13589.

    [3] Hu, H. J. et al., (2015). Obesity Alters the Microbial Community Profile in Korean Adolescents. PloS one, 10(7), e0134333.

    [4] Saha, S. et al., (2019). Microbial acclimatization to lipidic-waste facilitates the efficacy of acidogenic fermentation. Chemical Engineering Journal, 358, 188–196. https://doi.org/10.1016/j.cej.2018.09.220

Shotgun Metagenomic Sequencing

Shotgun Metagenomic Sequencing

Shotgun metagenomic sequencing based on NGS can comprehensively detect genes from all organisms, including uncultivable microorganisms or those in complex environments, providing information on microbial diversity and functional characteristics to aid in understanding relationships between microorganisms and their environments. It provides contig-level assembly of all organisms present in the sample, and gene annotation is performed by predicting CDS regions on the genome and utilizing databases such as UniProt, RefSeq, and Pfam.

Application

  • Identification of microbial community diversity in the environmnet
  • Metagenome assembled genomes

Sample/Laboratory Information

Sample/Laboratory Information
Sample Requirement gDNA > 10µg (minimum 5µg), 100ng/µl, DIN > 8
Library Kit Illumina
Sequencing Platform Illumina NovaSeq6000, PacBio Sequel Ⅱ
Recommended Sequencing Reads 70 million read pairs per sample

Bioinformatics

Workflow
Shotgun Metagenomic Sequencing

Standard Analysis

Remove host sequences

Metagenome assembled genomes

Prediction genomic region

Gene annotation

Taxonomy assign

Standard Analysis

Advanced Analysis

Classification of COG functional annotations

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Advanced Analysis
Full Length 16S rRNASequencing

Full Length 16S rRNASequencing

NGS-based microbiome research has the challenge of misclassifying at the species level due to the short read length, despite the advantage of low cost. PacBio's Single Molecule, Real-Time (SMRT) sequencing can decode approximately 1.5 Kbp in the 16S rRNA and 18S rRNA regions, providing highly accurate and high-resolution profiling results at the species level.

Application

  • Microbial taxonomic profiling
  • Full length 16S rRNA genomic analysis

Sample/Laboratory Information

Sample/Laboratory Information
Sample Requirement gDNA > 1µg (minimum 5µg), 100ng/µl, DIN > 8
Library Kit PacBio
Sequencing Platform PacBio Sequel Ⅱ
Recommended Sequencing Depth -

Bioinformatics

Workflow
Full Length 16S rRNASequencing
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