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Bioinformatics

Amplicon Metagenomic Sequencing Shotgun Metagenomic Sequencing Full Length 16S rRNA Sequencing

 Amplicon Metagenomic Sequencing

16S/18S/ITS Amplicon sequencing is a  technology that is more efficient and economical to identify and compare with bacteria and fungi present in a given sample. NGS-based analysis enables systematic taxonomic comparison of complex microbiome or cluster diversity in the environment,
which is difficult or impossible to cultivate, and helps researchers understand by providing visual and statistical indicators.
If it is not the case with amplicon sequencing using the universal primer set, sufficient consulting is required to conduct experiments and analysis thoroughly.

Workflow

BioinformaticsDetail View

Standard Analysis

Pick de novo OTUs

Taxonomic assignment

Diversity statistics (alpha-, beta- diversity)

Standard Analysis

Shotgun Metagenomic Sequencing

NGS-based shotgun metagenomic sequencing can comprehensively detect genes from uncultivated microorganisms or all organisms present in complex environments, and provides microbial diversity and functional characteristics to help recognize microbial relationships within environment and relationship between envrionment and microbial. All organisms present in the sample are assembled and provided at the library level.
It also enables providing an annotation such as UniProt, RefSeq, and Pfam after gene prediction.

Workflow
Shotgun Metagenomic Sequencing

BioinformaticsDetail View

Standard Analysis

Remove host sequences

Metagenome assembled genomes

Prediction genomic region

Gene annotation

Taxonomy assign

Standard Analysis

Advanced Analysis

Classification of COG functional annotations

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Advanced Analysis

Full Length 16S rRNASequencing

NGS-based microbiome research remains a big challenge to misclassify at the species level due to its short reading area while the cost burden is low.
PacBio's Single Molecule, Real-Time (SMRT) sequencing decodes about 1.5 Kbp in the 16S rRNA and 18S rRNA regions,
providing high-resolution profiling results with high accuracy at the species level.

Workflow
Full Length 16S rRNASequencing
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