Targeted Methylation Sequencing

Targeted Methylation Sequencing allows the detection of the methylation levels of the four nucleotides that can be methylated in genomic regions such as CpG islands and promoter regions.

It is possible to identify the methylation pattern of key regions using a smaller amount of sequencing data than whole-genome bisulfite sequencing. DNA methylation data plays a central role in studying biological phenomena such as gene expression, embryonic development, cell proliferation, differentiation, and chromosomal stability. Abnormal DNA methylation is also associated with DNA instability which can lead to human diseases such as cancer.

Bisulfite Sequencing

Bisulfite sequencing is a method used to determine the DNA methylation level of cytosine contexts (CpG, CHG, CHH) on the entire genome. It is used to study epigenetic phenomena at the molecular level and can identify differentially methylated regions between samples or groups. The genomic contents of these regions can be annotated to understand their impact on gene expression. Theragen Bio has conducted research on Platycosides biosynthesis using whole genome bisulfite sequencing data obtained from Doraji (a type of Korea ginseng).

ChIP Sequencing

ChIP sequencing is a sequencing method that combines chromatin immunoprecipitation (ChIP) with NGS and allows for the identification of DNA-protein binding sites through bioinformatics analysis. The identified binding sites can accurately predict the epigenetic state of each cell type and are essential for understanding the regulation of cellular processes, cell differentiation, or disease progression mechanisms. Since ChIP sequencing uses immunoprecipitation methods, there may inevitably be technical biases, but these can be reduced through precise experimental design using background controls such as IgG and computational analysis methods.