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Sample Requirements
CRITERIA | DNA | RNA | LIBRARY |
---|---|---|---|
Condition | Without degradation and RNA contamination | Without degradation and DNA contamination | Index information required |
Minimum Quantity | 1μg | 1μg | Vol 20μl |
Minimum concentration | 50ng/μl | 65ng/μl | 10ng/μl 10nmol |
Purity | OD260/280 = 1.5~2.2 | RIN ≥ 6.0 | If possible, Bioanalyzer result would be the best |
FFPE Requirement | Minimum Surface area = 25mm2 Minimum sample depth = 40microns Minimum sample volume = 1mm3 Minimum Nucleated cellularity = 80% Minimum tumor content = 20% |
Service Procedure
01
Project experiment/analysis design discussion
Check sample characteristics, library kit, platform, and amount analysis
02
Sample delivery
Send sample via FedEx/DHL
03
Sample QC
Checking amount, concentration, ratio, degradation, and other states using sample QC
04
NGS / Bioinformatics
Manufacturing library, sequencing and data analysis based on a prior discussed design
05
Data transfer
Sending raw data and analysis results of the corresponding sample
DNA / RNA Sample Shipping Guide
DNA Sample : 1ug (50ng/ul), OD260/280 = 1.6~2.0
RNA Sample : 1ug (65ng/ul), RIN value > 6.0
RNA Sample : 1ug (65ng/ul), RIN value > 6.0
NGS TotalOmics Sample Sheet
Step 1 : If there are QC results, write the sample information on the sample sheet of the order application.
Step 2 : Store samples in a deep freezer at -80°C before delivery.
Step 2 : Store samples in a deep freezer at -80°C before delivery.
Sample Packaging
01
A 1.5 ml tube is wrapped in para film to prevent the sealing sample from evaporating.
02
A sample sheet and labeled sample tube are sealed in a zipper bag.
03
Pack samples.
The thickness of packing box a minimum of 3.8 cm.
The thickness of packing box a minimum of 3.8 cm.
04
Surround the sample sufficiently by dry ice.
05
Tie a plastic bag well for sealing. (However, the plastic bag should not be tied too tightly in summer because it may burst.)
06
Fill the space between plastic bag and box with buffer materials such as newspaper and seal the box with tape.
Payment Guide
Basic Policy: Half of the total amount is paid in the data production stage and the remainder is paid within one month from the date when the NGS analysis results are sent.
Option: The total amount can be paid at one time before library construction.
* Payment Method
1. Tax Invoice
2. Credit Card
1. Tax Invoice
2. Credit Card
When you send the payment, transfer it to the account below.
Account No.: 120-147607-04-016
Bank Name: IBK
Account Name: Theragen Bio
Account No.: 120-147607-04-016
Bank Name: IBK
Account Name: Theragen Bio
Delivery Address
Theragen Bio
4th Fl. Korea Bio Park Bldg. C, 700 Daewangpangyo-ro
Bundang-gu, Seongnam-si, Gyeonggi-do
(Zip Code) 13488
4th Fl. Korea Bio Park Bldg. C, 700 Daewangpangyo-ro
Bundang-gu, Seongnam-si, Gyeonggi-do
(Zip Code) 13488
Delivery Inquiries
Tel: 82-31-888-9313
E-mail: totalomics@theragenbio.com
Tel: 82-31-888-9313
E-mail: totalomics@theragenbio.com